ANO6 and neoplasm: To assess the effects of TMEM16F knockout on tumor growth and the tumor microenvironment and compare effects to those of the Xkr8 knockout model described above (Fig. 2B–D), EO771 WT cells or EO771 TMEM16F KO cells were transplanted orthotopically into the mammary gland fat pads of C57BL/6 WT mice.