The succinylome is principally fueled by tricarboxylic acid (TCA) cycle-derived succinyl-CoA, with its spatiotemporal dynamics governed through balanced enzymatic control via succinyltransferases and desuccinylases.34 Here, we observed the global succinylation hyperactivation in HCC, which is consistent with previous reports.35,36 Given these findings, we employed LC-MS/MS proteomic profiling to systematically identify SRSF11 as a conserved succinylation substrate across HCC cell lines, followed by validation in patient-derived tissues. This evidence concerns the gene SRSF11 and hepatocellular carcinoma.