CDKN2A and liver cancer: To further validate the relationship between TMBIM1 and senescence, we performed immunohistochemical staining of cancerous tissue from patients with liver cancer and the statistical analyses revealed the negative correlation between TMBIM1 and senescence markers cyclin-dependent kinase inhibitor 1A (P21) and cyclin-dependent kinase inhibitor 2A (P16) (Fig. 3C).