Of them, 10 aging-related CpG sites were mapped to 6 genes (i.e., TNF, BICC1, TBX3, SUCNR1, NCF2, DIP2B), and the altered methylation of cg03037030 located in TNF was associated with the risk of CRC, colon cancer, and rectal cancer, with consistent effect direction (Fig. 3, Supplementary Table 22). Here, TBX3 is linked to rectal cancer.