The percentages and total numbers of Helios+ migratory natural gut-homing Th17-like Tregs in mLNs were much lower in NOD.LckCrePrdm1F/F mice than in B6.LckCrePrdm1F/F mice (Fig. 3j), supporting that the impairment of splenic Blimp-1-deficient nTreg migration toward colonic inflammatory sites in diabetes-prone mice compared with non-diabetes-prone mice. This evidence concerns the gene PRDM1 and diabetes mellitus.