Additionally, the expression of NF-κB pathway-related genes (Rel, Rela, Ikbkb, Nfkbie, and Nfkb1) were lower in Blimp-1-deficient Tregs in NOD than in C57BL/6 mice (Fig. 4a), consistent with previous reports that NF-κB c-Rel ablation in Tregs impaired their suppressive function and markedly reduced melanoma growth.42 This evidence concerns the gene PRDM1 and melanoma.