Using targeted panel sequencing for 1021 genes, we analyzed gene mutation frequency and genetic mutual relationship of ten canonical cancer signaling pathways, namely Cell Cycle, Hippo, MYC, NOTCH, NRF2, PI3Kinase/Akt, RTK-RAS, TGF-β, TP53, and β-catenin/WNT signaling. The gene discussed is TP53; the disease is cancer.