We found that HG stimulation resulted in high expression of miR-146b-5p in HBZY-1 cells, downregulation of the target protein Merlin, and inhibition of the Lats1 and MST1 proteins, which led to the intranuclear accumulation of YAP and the deposition of α-SMA during DKD progression. This evidence concerns the gene LATS1 and diabetic kidney disease.