To elucidate the mechanism underlying IRE+Combo-induced eradication of s.c. 3LLOVA lung cancer, we also analyzed single cell suspensions enzymatically prepared from IRE-, IRE+CpG/pIC/PD-L1 Ab- and IRE+Combo-treated 3LLOVA cancer tissues by flow cytometry, as we previously described (23). Here, CD274 is linked to cancer.