CSF3 and chronic myelogenous leukemia, BCR-ABL1 positive: To evaluate in-vitro cytotoxicity, PBHCs mobilized with either G-CSF alone or G-CSF + ISO were co-cultured with luciferase-tagged K562 cells —a chronic myeloid leukemia cell line sensitive to NK cell cytolysis—at varying effector-to-target (E: T) ratios for 24 h.