To identify if these receptors are involved in initiating the necroptotic pathway during HCMV infection of monocytes, we used a neutralizing antibody against TNFR1 (NAb TNFR1), the TLR4-selective inhibitor TAK-242 (iTLR4), or the TLR3-selective inhibitor (R)-2-(3-chloro-6-fluorobenzo[b]thiophene-2-carboxamido)-3-phenylpropanoic acid (iTLR3). The gene discussed is TLR4; the disease is cytomegalovirus infection.