For further investigation, we detected the expression of CPNE3 in NSCLC cell lines and found that the expression of CPNE3 in lung cancer cell lines (HCC827, PC‐9, H1299, A549, H1650, H460, H1703, H226 and H520) was higher than that in a normal lung epithelial cell line (BEAS‐2B) using western blotting and qRT–PCR (Figure 1J). The gene discussed is CPNE3; the disease is lung carcinoma.