In WT systems (Meng et al., 2014), cancer-associated mutations (P114L, R116K, R166H, G309A, and R316W) that influence protein–mRNA interaction (Figure 4b) showed a stable RMSF, specifically in the closed conformation of yeast eIF4A (structurally similar to human eIF4A3) upon binding ATP and mRNA. The gene discussed is EIF4A3; the disease is cancer.