We next immunoblotted each biochemical fraction from all patients for NSUN1 and observed that in most patients (control and ALS/FTD), NSUN1 remained predominantly in the most soluble (low-salt) fraction (Fig 8C), consistent with the absence of insoluble aggregation with TDP-43 in ALS spinal cord. This evidence concerns the gene NOP2 and amyotrophic lateral sclerosis.