For the breast cancer specimen (estrogen receptor [ER]-negative, PGR-negative, and HER2-positive: see Figure S4A for more details), four regions were defined by the pathological diagnosis based on the H&E images, including two nontumor regions (R1 and R2) and two tumor regions (R3 and R4) (Figure 4A). Here, ESR1 is linked to neoplasm.