Validation experiments using qRT-PCR and Western blotting showed significantly elevated FMR1 expression in human gastric cancer cell lines (MKN-74, HGC-27, SNU-484, AGS) compared with the normal gastric epithelial cell line GES-1 (P < 0.001), with the highest expression in SNU-484 cells (Fig. 1D,E). The gene discussed is FMR1; the disease is gastric cancer.