Higher FPKM values for FADS1, ELOVL5, and ACLY were detected in cancer tissues than in adjacent normal tissues (P < 0.01; P < 0.01; P < 0.01), suggesting that alterations in lipid metabolism in ESCC are mediated by altered expression of these genes (Figure S2C). This evidence concerns the gene ACLY and esophageal squamous cell carcinoma.