YAP1 and hepatocellular carcinoma: [22] To investigate the genetic landscape of the liver following Hippo pathway abnormalities, we first constructed a hydrodynamically transfected spontaneous HCC model driven by abnormal expression of c-MYC/AKT/vector or c-MYC/AKT/YAP via hydrodynamic transduction (Figs.1A, B and S1A, B).