[22] To investigate the genetic landscape of the liver following Hippo pathway abnormalities, we first constructed a hydrodynamically transfected spontaneous HCC model driven by abnormal expression of c-MYC/AKT/vector or c-MYC/AKT/YAP via hydrodynamic transduction (Figs.1A, B and S1A, B). The gene discussed is AKT1; the disease is hepatocellular carcinoma.