UBA3 and neoplasm: The analyses showed that FP-RMS cell lines were the most dependent on UBA3 expression and one of the most dependent cell lines on NAE1 expression compared to different adult and pediatric tumor cell lines and to FN-RMS as well (Chronos score −0.70 and −0.56 for NAE1 and −0.87 and −0.69 for UBA3 in FP-RMS and FN-RMS cells, respectively, with Chronos score < −0.5 is considered a significant dependency) (Fig. 1A, B).