Initial BODIPY fluorescence staining revealed significantly greater lipid droplet accumulation in SDHB knockout (KO) GIST-T1 cells than in wild-type GIST-T1 cells (negative control, NC) (Fig. 4a), indicating increased lipid uptake after SDHB knockout and that tumor cells with SDHB deficiency may rely more on energy from fatty acid metabolism for growth. This evidence concerns the gene SDHB and gastrointestinal stromal tumor.