TP53 and infection: In this study, we combined transcriptome analyses with experimental validation in p53-positive A549 and p53-negative H1299 cells infected with HAdV-C5 and E1B-55K-deficient mutant (ΔE1B-55K) to investigate E1B-55K-dependent gene expression patterns during infection and in the context of IFN-alpha (IFN-α) stimulation.