t-SNE dimensionality analysis revealed that CD8+ T cells from the peripheral blood of pMMR CRC patients and healthy individuals clustered together, topographically distinct from CD8+ T cells originating from non-tumor colon tissues and tumor tissues in our dataset (Fig. 3a, left) and public dataset GSE108989 (Fig. 3b, left). The gene discussed is CD8A; the disease is colorectal carcinoma.