Most relevant to this study, calpain-2, a classical calpain,plays a vital role in maintaining mammary gland homeostasis and inthe progression of BC via various well-characterized signaling pathways.−,  In line with this, RNAi knockdown of calpain-2 in the AC2M2 mousemammary carcinoma cell line reduced growth and migration in vitroand also decreased tumor growth in vivo using a mammary fat pad engraftmentmodel. The gene discussed is CAPN2; the disease is breast cancer.