These single-cell and spatial criteria delineate an ovarian cancer ecosystem in which TGF-β–responsive fibroblast matrices, altered antigen presentation, and checkpoint-dominated contact zones converge to produce immune exclusion; this phenotype maps onto the broader TGF-β barrier framework defined in other indications and provides tractable readouts for risk stratification and therapeutic testing. Here, TGFB1 is linked to ovarian carcinoma.