PYGB and cancer: To delineate the relationship between NRGs and immune infiltration in LUSC, mRNA expression patterns of CAMK2A, CHMP4C, and PYGB were systematically correlated with immune-related gene sets, including chemokines, chemokine receptors, major histocompatibility complex (MHC) molecules, immunoinhibitors, and immunostimulators, across 32 TCGA cancer types (Fig. 11A).