In addition, the m5C reader ALYREF regulates HCC by directly binding to the 295 HCC cell cycle and apoptosis-related target genes, including the binding of ALYREF to the m5C site in EGFR 3′-UTR to stabilize EGFR mRNA, thereby activating the STAT3 pathway. This evidence concerns the gene STAT3 and hepatocellular carcinoma.