Pseudomonas species dominated in the constructed metatranscriptome with the highest number of species (205) and expressed genes (3898), including the highest number of virulence (1952), secretory (198), peptidases (231) and antimicrobial resistance genes (44), highlighting a significant role of Pseudomonas species in bovine mastitis pathogenesis. The gene discussed is LAP3; the disease is Bovine mastitis.