In TISF-DNA, we found mismatch repair (MMR) genes (MSH2, MSH3, MSH6, MLH1, MLH3, PMS1, PMS2), and temozolomide-related high mutations [the accumulation of G: C>A: T: transitions at non-CPg sites in hypermutated gliomas after exposure to alkylating Agents (20, 21)]. This evidence concerns the gene MLH1 and central nervous system cancer.