In this study, we used the CRISPR-Cas9 system to identify such tumor dependent genes in TNBC cells, including TONSL, TIMELESS, RFC3, and RAD51. These genes are primarily involved in DNA replication and repair, suggesting that TNBC tumor cells exhibit active DNA replication while potentially experiencing DNA mismatches and damage (18–20). This evidence concerns the gene TIMELESS and neoplasm.