The KEGG classification results revealed that 194 signaling pathways were enriched according to the 261 DEGs, and cancer‐related signaling pathways primarily included pathways in cancer, proteoglycans in cancer, VEGF, MAPK, Ras, GnRH, mTOR, Wnt, FoxO, PI3K‐Akt, and MicroRNAs in cancer, indicating that the inhibitory efficacy of TMF on HeLa tumors was involved in multipathing in vivo (Figure 4C,D). This evidence concerns the gene AKT1 and cancer.