Proteomics analysis identified 19 differentially expressed proteins (DEPs) from HeLa cancer cells after TMF treatment, including downregulation of HSP60, sTNF‐R1, JNK, TAK1 (S412), TBK1 (S172), ZAP70 (Y292), ATF2, c‐Fos, c‐JUN, Smad1, Smad5, and Stat6 (Tyr64), alongside upregulation of sTNF‐R2, AKT, GSK3b, MKK3, MKK6, MSK2, and P38. This evidence concerns the gene SMAD1 and cancer.