To further investigate the anti-tumor efficacy of CAR-T cells and understand the underlying mechanisms, we performed ELISA experiments to measure the release of key cytokines, including IL-2, IL-6, IL-10, TNF-α, GM-CSF, perforin, and granzyme B from the supernatant of CAR-T cells and NT cells co-cultured with tumor cells at an E:T ratio of 2:1 for 24 h. The gene discussed is TNF; the disease is neoplasm.