CCNA2 and infection: Because of the effects of MTX on mitosis, we next analyzed the transcript levels of cell cycle regulators and found that, in untreated Cg>SPE-Act whole larvae, Cyclin A, Cyclin B and Cyclin D were substantially upregulated, and that MTX treatment attenuated their expression (Fig. 3G1–G3,H1–H3), like we had observed during infection.