To explore the potential link of these S100A8+ macrophages and CD36 expression in hepatocytes in MASH livers from patients, we performed coimmunostaining with antibodies against CD36, Heppar1 (a hepatocyte marker), and IBA1 (a macrophage marker), and found that CD36 expression was induced in both hepatocytes and macrophages (Supplemental Figure 18). This evidence concerns the gene S100A8 and metabolic dysfunction-associated steatohepatitis.