Adipocyte-specific overexpression of the Bcl2 gene mitigated HFD-induced steatosis and MASLD progression in mice, accompanied by notable alterations in the hepatic infiltration of macrophages, particularly a population of S100A8+ macrophages identified via single-cell RNA-Seq (scRNA-Seq) analysis. This evidence concerns the gene BCL2 and metabolic dysfunction-associated steatotic liver disease.