The most complex model (model 1) including whr, ALT, GGT, 1-deoxySO, 1-deoxySA, C17SA, MeC18SO, alanine, serine, asparagine and histidine as independent variables (Fig. 1A) showed with 0.851 (p = 8.18E-29) the highest area under the curve (AUC) resulting in a sensitivity of 70.0% and a specificity of 84.5% for the distinction between MASH and no-MASH. This evidence concerns the gene GPT and metabolic dysfunction-associated steatohepatitis.