To validate the potential role of the circANKRD52/PRKACB axis in melanoma, sh‐PRKACB was further introduced in the oe‐circANKRD52‐transfected A375 cells, or oe‐PRKACB was introduced in the sh‐circANKRD52‐transfected SK‐MEL‐28 cells, and the transfections were confirmed by RT‐qPCR (Figure S4). The gene discussed is PRKACB; the disease is melanoma.