To determine whether the IFN-independent viral induction of LGP2 also operates via pathways other than TLR3, we used SeV infection and cytoplasmic delivery of poly(I:C) by Lipofectamine transfection (t-pIC), respectively, to engage the cytosolic RLRs, RIG-I and MDA5, and assessed the downstream antiviral response (Fig. 2b; quantification data presented in Fig. S2). The gene discussed is IFIH1; the disease is infection.