Consistent with the slower tumor growth in the triple therapy + LC cohort, we measured increased cytotoxic CD4+GrB+, CD4+, and CD8+ IFNγ+TNFα+ and a trend to more memory T cells and fewer CD8+ regulatory T cells (Tregs) in the tumors (Fig. 1C; Supplementary Fig. S3A). This evidence concerns the gene CD8A and laryngotracheoesophageal cleft.