AFP and hepatocellular carcinoma: Supplementary Figure S2 is the detailed flowchart of this study. Among them, 75 (41.4%) were GPC3-negative HCC, and 106 (58.6%) were GPC3-positive HCC. Then, the comparison of clinical variables was performed between the two groups, as shown in Table 1. AFP ≥ 10 ng/mL, age, HBsAg-negative, gender-female, AST, NLR, ANRI, and MLR were more correlated with GPC3 expression (p<0.2). Lastly, as presented in Table 2, the multivariate logistic regression identified four variables as independent predictors for GPC3-positive (p<0.05) (Figure 2).