In in vivo assessments, compared with the model group, YQHXJDD ELNVs significantly inhibited ovarian cancer growth and metastasis, increased tumor necrosis factor-α (TNF-α) levels in mouse peripheral blood, and positively regulated the M1/M2 macrophage ratio and CD4+/CD8+ T cell ratio in omental tissues (all P < 0.05). This evidence concerns the gene TNF and ovarian carcinoma.