Flow cytometric analysis revealed that YQHXJDD ELNVs had no significant effect on the proportions of splenic M1-polarized macrophages (with pro-inflammatory and anti-tumor properties), NK cells, and effector T cells, as well as the expression of functional markers (Granzyme B, IFN-γ) in effector T cells and NK cells (all P ≥ 0.05). The gene discussed is IFNG; the disease is neoplasm.