TP53 and head and neck squamous cell carcinoma: As a result, a study by Marcozzi and colleagues established that RCA-coupled nanopore sequencing achieved a detection limit of 0.02% VAF for TP53 mutations in ctDNA, permitting serial assessment of cancer dynamics through mutation quantification in head and neck squamous cell carcinoma (HNSCC) (Marcozzi et al., 2021).