Strikingly, ACACA expression exhibited significant negative correlations with the immune checkpoint-related genes (PDCD1, LAG3, LAGLS9, IDO1, CD244, CTLA4 and TIGIT), while showing positive associations with other genes (TGFBR1, KDR, IL10RB, CD160 and CD274) across most cancer types (Figure 3D). This evidence concerns the gene LAG3 and cancer.