While the prior study identified M2I‐1 as a small molecule that inhibits the MAD2L1‐CDC20 interaction in vitro, its characterization was limited to this biochemical activity.[44] We not only confirmed the direct binding of A2 to MAD2L1 but also demonstrated its potent tumor‐suppressive effects across cellular models, PDOs, and CDX/PDX models. This evidence concerns the gene MAD2L1 and neoplasm.