Pooled analyses assessed Raman spectroscopy’s ability to: (1) delineate tumor margins; (2) grade high-grade (HGG) versus low-grade gliomas (LGG); (3) distinguish astrocytomas from oligodendrogliomas; (4) classify IDH-wildtype (IDHwt) versus IDH-mutant (IDHmut) gliomas; and (5) discriminate gliomas from other brain tumors. From 206 studies, 19 were used in pooled analyses. Here, IDH1 is linked to central nervous system cancer.