Here, we demonstrated a reduced migratory and colony-forming potential of hepatocellular and cholangio-carcinoma cell lines in vitro and impaired tumour-forming ability in vivo associated with inhibition of CD44v6, along with an upregulation of cancer stem cell-related genes (OCT3/4, NANOG) upon overexpression of CD44v6. The gene discussed is NANOG; the disease is cancer.