AKT1 and neoplasm: This finding is also consistent with prior research on T cells, which demonstrated that ATP may directly drive phosphatidylinositol 3,4,5-trisphosphate (PIP3) production, thereby establishing a positive feedback loop in PI3K/AKT signaling.42,43 Specifically, since ATP cannot freely diffuse across the cell membrane and is generated primarily through intracellular glycolysis and oxidative phosphorylation, the intracellular ATP pool varies among different tumor subpopulations.