The role of Th1/Th17 imbalance driving CRS and neutropenia is verified with single-cell RNA-seq, where similar molecular characteristics were observed after adoptive transfer of Th1 cells with IFN-γ KO CAR-T compared with WT CAR-T cells alone and IFN-γ KO CAR-T treatment compared with adoptive transfer of Th17 cells. The gene discussed is IFNG; the disease is neutropenia.