Prior work has established that the Tie2 antagonist, angiopoietin‐2 (Ang‐2), suppresses collateral vessels in hindlimb ischemia.[9] We demonstrate that a single treatment with Vasculotide can enhance arteriogenesis, providing the first evidence that the Ang‐1 peptide agonist influences collateral vessels in ischemic stroke. Here, TEK is linked to ischemic stroke.