Previous studies have reported that chromatinstates modulate nuclear elasticity and that serum influences thesechromatin states.−,  To investigate the effects of serum depletion onnuclear elasticity, we measured the elasticity of the intact nuclearsurface in human lung cancer cells (PC9, harboring the EGFR Δexon19)., Figure a illustrates the NE-AFM method used for these measurements,where 256 force curves were taken at 16 × 16 arrayed-xy positions over a 1 × 1 μm2 areaaround the nucleus center. This evidence concerns the gene EGFR and lung carcinoma.