To further investigate the regulation of BECN1 release by ZNF460, a siRNA targeting ZNF460 was used, which reduced the expression of ZNF460 in CRC cells (Supplementary Figure S3D), and the western blotting results revealed that BECN1 was significantly reduced after ZNF460 knockdown after irradiation in CRC cells (Figure 5D). Here, ZNF460 is linked to colorectal carcinoma.