In our study, elevated PF4 levels in the supernatant of platelet-stimulated cultures were accompanied by increased TGF-β1 expression at both the transcript and protein level, along with upregulation of mesenchymal and EMT-related transcriptional markers, further strengthening the link between platelet-derived PF4, TGF-β activation, and EMT-like changes in kidney injury. Here, PF4 is linked to kidney injury.