Intriguingly, a trend toward reduced expression of NLRP3 (NOD-, LRR-, and pyrin domain-containing protein 3), a marker of inflammation, was observed in mt-EcGAPR mice compared with that in C57BL/6 J control mice, although the difference was not statistically significant, suggesting the dominance of the ER stress–ATF6–pyroptosis axis in the early stages of glaucoma (Supplementary Fig. 7h, i). Here, NLRP3 is linked to glaucoma.